Development of rigorous biological procedures

Rigorousness in biological experiments is an essential requirement for the comparison with other experiments, for the replication of the same and also for the adequate interpretation of the results obtained.
To this end, cell models must be selected in accordance with the biological parameters considered, the biological assays have to be developed in the specific laboratory conditions; treatments with agents inducing specific changes in the parameters under investigation (positive controls) should be included to guarantee that cells are able to respond adequately to stimuli, and that the biological assays are properly applied.